iScript™ Advanced cDNA Synthesis Kit, 25 x 20 µl rxns from Bio-Rad

Product Specs
ItemiScript™ Advanced cDNA Synthesis Kit, 25 x 20 µl rxns
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1725037
Brand/TrademarkLSG GXD

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) IFIH1 (MDA5) is required for innate immune detection of intron-containing RNA expressed from the HIV-1 provirusbioRxivDecember 12, 2023Mehmet Hakan Guney, Karthika Nagalekshmi, Sean Matthew McCauley, Claudia Carbone, Ozkan Aydemir, Jeremy Lubanby NanoDrop 2000 (Thermo), were used to make cDNA with iScript Advanced cDNA Synthesis Kit (BioRad, 1725037). cDNA was amplified by qPCR with iTaq Universal SYBR Green Supermix (BioRad, 1725121), using HIV-1-specific

(2) The Ultraviolet Irradiation of Keratinocytes Induces Ectopic Expression of LINE-1 Retrotransposon Machinery and Leads to Cellular SenescenceBiomedicinesNovember 10, 2023Fadi Touma, Marine Lambert, Amelia Martínez Villarreal, Jennifer Gantchev, Brandon Ramchatesingh, Ivan V. Litvinovng) was retrotranscribed to synthesize cDNA with an iScript™ Advanced cDNA Synthesis Kit (BioRad Cat# 1725037) for subsequent use in qPCR. qPCR was performed using SsoAdvanced Universal SYBR® Green Supermix (BioRad

(3) A rapid, multiplex digital PCR assay to detect gene variants and fusions in non‐small cell lung cancerMolecular OncologyNovember 1, 2023Bryan Leatham, Katie McNall, Hari K. K. Subramanian, Lucien Jacky, John Alvarado, Dominic Yurk, Mimi Wang, Donald C. Green, Gregory J. Tsongalis, Aditya Rajagopal, et al.converted to cDNA using the Bio‐Rad cDNA Synthesis Kit according to the manufacturer's instructions (PN 1725037). cDNA was then quantified by Qubit4 (Thermo Fisher Scientific). Three ddPCR reactions were set up for

(4) The tectonic complex regulates membrane protein composition in the photoreceptor ciliumNature CommunicationsSeptember 13, 2023Hanh M. Truong, Kevin O. Cruz-Colón, Jorge Y. Martínez-Márquez, Jason R. Willer, Amanda M. Travis, Sondip K. Biswas, Woo-Kuen Lo, Hanno J. Bolz, Jillian N. Pearringsynthesized using 500 ng of total RNA per biological sample using iScript Advanced cDNA Synthesis (1725037; Bio-Rad, Hercules, CA). Quantitative PCR was performed on 1 µl of cDNA per biological sample using

(5) Investigation of mesalazine as an antifibrotic drug following myocardial infarction in male micePhysiological ReportsSeptember 9, 2023Stephan R. Künzel, Luise Winter, Maximilian Hoffmann, Theresa A. Kant, Jessica Thiel, Romy Kronstein‐Wiedemann, Erik Klapproth, Kristina Lorenz, Ali El‐Armouche, Susanne Kämmerer(cat. no. 74004, Qiagen). cDNA was synthesized using the iScript Advanced cDNA synthesis kit (cat. no. 1725037, Bio‐Rad Laboratories GmbH). CFX manager software (Bio‐Rad Laboratories GmbH) was used for data analysis. 2.6

(6) Praziquantel inhibits Caenorhabditis elegans development and species-wide differences might be cct-8-dependentPLoS OneAugust 10, 2023Janneke Wit, Clayton M. Dilks, Gaotian Zhang, Karen S. Kim Guisbert, Stefan Zdraljevic, Eric Guisbert, Erik C. Andersensynthesized into cDNA using first-strand synthesis (Bio-Rad iScript Advanced cDNA Synthesis Kit for RT-qPCR, 1725037). qPCR was then performed as previously described [32] using custom primers (S1 Table). Amplification

(7) Retinoic acid is dispensable for meiotic initiation but required for spermiogenesis in the mammalian testisDevelopmentJuly 15, 2023Oleksandr Kirsanov, Taylor A. Johnson, Bryan A. Niedenberger, Taylor N. Malachowski, Benjamin J. Hale, Qing Chen, Brad Lackford, Jiajia Wang, Anukriti Singh, Karen Schindler, et al.qRT-PCR analyses, 0.5 μg total RNA was reverse-transcribed using the iScript cDNA Synthesis Kit (Bio-Rad, 1725037). qPCR was performed using the SsoFast EvaGreen Supermix (Bio-Rad, 1725201) on the Bio-Rad CFX-384 or

(8) Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombinationSTAR protocolsMarch 17, 2023Weidenhammer LB, Liu HQ, Luo L, Williams NT, Deland K, Kirsch DG, Reitman ZJ.KIT0103 Direct-zol RNA Kit,Zymo Research,Cat# R2051 iScript™ Advanced cDNA Synthesis Kit,Bio-Rad,Cat# 1725037 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, UMNSAH/DF-1

(9) Cotargeting of BTK and MALT1 overcomes resistance to BTK inhibitors in mantle cell lymphomaJournal of Clinical InvestigationFebruary 1, 2023Vivian Changying Jiang, Yang Liu, Junwei Lian, Shengjian Huang, Alexa Jordan, Qingsong Cai, Ruitao Lin, Fangfang Yan, Joseph McIntosh, Yijing Li, et al.described elsewhere (30). Briefly, first-strand cDNA was synthesized via the iScript cDNA Synthesis Kit (1725037, Bio-Rad) according to the manufacturer’s manual. Quantitative real-time PCR was conducted using SsoAdvanced Universal SYBR Green Supermix

(10) The short-chain fatty acid butyrate exerts a specific effect on VE-cadherin phosphorylation and alters the integrity of aortic endothelial cellsFrontiers in cell and developmental biologyJanuary 1, 2023Guo J, Terhorst I, Stammer P, Ibrahim A, Oberhuber A, Eierhoff T.100 ng of total RNA was reverse-transcribed using the iScript™ Advanced cDNA Synthesis Kit (Bio-Rad, 1725037) at 46°C for 20 min and 95°C for 1 min in a thermal cycler (Mastercycler nexus gradient, Eppendorf).

(11) Nucleotide- and Protein-Dependent Functions of Actg1Molecular Biology of the CellAugust 1, 2022Lauren J. Sundby, William M. Southern, Katelin M. Hawbaker, JesÃºs M. Trujillo, Benjamin J. Perrin, James M. ErvastiNanoDrop 1000 spectrophotometer (ThermoFisher Scientific). A Bio-Rad iScript Advanced cDNA synthesis kit (1725037) was used to synthesize first-strand cDNA from a standard amount of RNA. Isoactin and Actbc–g control

(12) PEAPOD repressors modulate and coordinate developmental responses to light intensity in ArabidopsisNew PhytologistAugust 1, 2022Derek W. R. WhiteRNA, using both random hexamer and oligo(dT)18 primers and iScript Advanced Reverse Transcriptase (1725037; Bio‐Rad) according to the manufacturer's protocol. Quantitative PCR (qPCR) analyses were undertaken

(13) Skeletal muscle MiR-210 expression is associated with mitochondrial function in peripheral artery disease patientsTranslational research : the journal of laboratory and clinical medicineAugust 1, 2022Ismaeel A, Fletcher E, Miserlis D, Wechsler M, Papoutsi E, Haynatzki G, Smith RS, Bohannon WT, Koutakis P.cDNA was synthesized using an iScript Advanced cDNA synthesis Kit (Bio-Rad Laboratories, Hercules, CA, 1725037). PCR reactions used the following primers: COX10 (Integrated DNA Technologies, Coralville, IA, Hs.PT.58.600173),

(14) Nobiletin Attenuates Anesthesia/Surgery-Induced Neurocognitive Decline by Preserving the Expression of Clock Genes in MiceFrontiers in NeuroscienceJuly 7, 2022Zhuonan Sun, Ning Yang, Xixi Jia, Yanan Song, Dengyang Han, Xiaoxiao Wang, Jie Sun, Zhengqian Li, Zhiyi Zuo, Xiangyang GuoNanoDrop 2000 (Thermo, Waltham, MA, China). The High Capacity cDNA Reverse Transcription Kit (Cat. NO. 1725037; Bio-Rad) was used to synthesize cDNA, and SYBR green real-time PCR was performed using the Applied

(15) Association of the receptor for activated C-kinase 1 (RACK1) with ribosomes in Plasmodium falciparumJournal of Biological ChemistryJune 1, 2022Jessey Erath, Sergej Djuranovic50 ng of total RNA was used to generate cDNA via the iScript Advance cDNA Kit for RT-qPCR (BioRAD #1725037), for each sample including no reverse transcriptase and no template controls following the manufacturer’s

(16) Analysis of brain region-specific mRNA synthesis and stability by utilizing adult mouse brain slice cultureSTAR ProtocolsJune 1, 2022Volodymyr Dzhala, Alan J. Fowler, Britt A. DiMarzio, Kevin J. Staley, Jaehong Suhassays,Critical commercial assays ,, iScript Advanced cDNA Synthesis Kits for RT-qPCR,Bio-Rad,Cat# 1725037 SsoAdvanced Universal Probes Supermix,Bio-Rad,Cat# 172-5280 Click-iT Nascent RNA Capture Kit,Thermo Fisher Scientific

(17) Telmisartan attenuates human glioblastoma cells proliferation and oncogenicity by inducing the lipid oxidationAsia-Pacific Journal of Clinical OncologyJune 1, 2022Yan Wang, Tengrui Zhang, Chen Li, Jia Guo, Baohui Xu, Lixiang Xue79254; Qiagen) for 30 min at room temperature. The High Capacity cDNA Reverse Transcription Kit (Cat# 1725037; Bio‐Rad) was used to synthesize cDNA, and SYBR green real‐time PCR was performed using MyCycler Thermal Cycler

(18) High-Fat Diet Augments the Effect of Alcohol on Skeletal Muscle Mitochondrial Dysfunction in MiceNutrientsFebruary 28, 2022Ismaeel A, Laudato JA, Fletcher E, Papoutsi E, Tice A, Hwa LS, Miserlis D, Jamurtas AZ, Steiner J, Koutakis P.USA), followed by cDNA synthesis using an iScript Advanced cDNA Synthesis Kit (Bio-Rad Laboratories, 1725037, Hercules, CA, USA). Real-time PCR was used to determine the relative expression of the following antioxidant

(19) Pentagalloyl glucose inhibits TNF‐α‐activated CXCL1/GRO-α expression and induces apoptosis‐related genes in triple-negative breast cancer cellsScientific ReportsDecember 1, 2021Patricia Mendonca, Sumaih Alghamdi, Samia Messeha, Karam F. A. SolimanPrimers for RT-PCR, SsoAdvanced Universal SYBR (Cat# 1725271), iScript Advanced cDNA Synthesis Kit (Cat# 1725037), apoptosis PCR arrays (Cat# 10034106) were purchased from Bio-Rad (Hercules, CA, USA). Western assays

(20) Klf5 establishes bi-potential cell fate by dual regulation of ICM and TE specification genesCell reportsNovember 9, 2021Kinisu M, Choi YJ, Cattoglio C, Liu K, Roux de Bezieux H, Valbuena R, Pum N, Dudoit S, Huang H, Xuan Z, et al.15596). cDNA was reverse-transcribed using iScript Advanced Reverse-Transcriptase (Bio-Rad, Cat. # 1725037). All real-time qPCR analyses were performed using SYBR FAST qPCR Master Mix (Kapa Biosystems, Cat.

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